张璐;谢玉波;陈燕桦;卢伊芝;何芳;梁蓓薇;

• 1:广西医科大学第一附属医院

摘要(Abstract)：

目的观察体外循环(CPB)期间静脉泵注右美托咪定(Dex)的大鼠海马CA1区神经细胞凋亡情况、脑皮质组织IL-6表达变化,并探讨其机制。方法 48只SPF级成年健康雄性SD大鼠随机分为3组各16只。Dex组(D组)在制模前10 min按照5μg/kg的负荷剂量静脉泵注Dex,后制备CPB模型,制模后转CPB机2 h,转流期间Dex以5μg/(kg·h)的泵注速度维持,转流2 h后停CPB机;体外循环组(C组)建立大鼠CPB模型,模型制备方法参照D组,制模后转CPB机2 h,转流期间给予与D组等量生理盐水;假手术组(S组)只作动静脉血管穿刺置管,不转CPB机,给予与D组等量生理盐水。CPB转流结束后立即处死各组8只大鼠,取脑组织,TUNEL法检测海马CA1区凋亡神经细胞,计算神经细胞凋亡率。CPB转流2 h结束后立即处死各组余下的8只大鼠,冰上分离皮质和海马组织,ELISA法检测皮质组织匀浆上清液白细胞介素6(IL-6),Western blot法检测海马组织磷酸化Janus激酶2(pJAK2)、磷酸化STAT3(pSTAT3)蛋白。结果与S组比较,C组大鼠海马CA1区神经细胞凋亡率、皮质组织匀浆上清液IL-6水平及海马组织pJAK2、pSTAT3蛋白相对表达量升高(P均<0.05);与C组比较,D组大鼠海马CA1区神经细胞凋亡率、皮质组织匀浆上清液IL-6水平及海马组织pJAK2、pSTAT3蛋白相对表达量降低(P均<0.05)。结论 CPB期间静脉泵注Dex可减轻大鼠神经细胞凋亡和脑部炎症,机制可能与其可抑制JAK2/STAT3信号通路的激活有关。

关键词(KeyWords)： 右美托咪定;体外循环;白细胞介素6;神经细胞;Janus激酶2;信号转导子和转录激活子3

Abstract:

Keywords:

基金项目(Foundation): 广西重点研发计划项目(桂科AB18221031);; 广西自然科学基金项目(2018GXNSFAA294007)

作者(Author): 张璐;谢玉波;陈燕桦;卢伊芝;何芳;梁蓓薇;

Email:

DOI:

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