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目的 观察代谢相关脂肪性肝病（MAFLD）小鼠肝组织盘状结构域受体2(DDR2)的表达变化。方法 将10只8周龄雄性C57BL/6J小鼠随机分为普通饲料饮食组（NC组）和高脂饮食组（HFD组），每组5只。HFD组给予高脂饲料喂养，NC组给予普通饲料喂养，共16周。禁食12 h后取两组小鼠，称重后眼眶取血，检测血清肝功能[丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）]及血脂[总胆固醇（TC）、甘油三酯（TG）]，取肝组织称重后测算肝脏指数，采用酶法检测肝组织TG、游离脂肪酸（FFA）,HE染色和油红O染色后观察肝组织病理变化，分别采用qPCR和Western blotting法检测肝组织DDR2 mRNA及蛋白。结果 与NC组比较，喂养16周后HFD组体重大（P<0.05）。HFD组血清ALT、AST、TC及TG水平均高于NC组（P均<0.05）。HFD组肝脏重量、肝脏指数及肝脏TG、FFA含量均高于NC组（P均<0.01）。与NC组比较，HFD组肝组织出现明显脂肪变性。HFD组肝组织DDR2 mRNA和蛋白相对表达量均高于NC组（P均<0.01）。结果 MAFLD小鼠肝组织DDR2 mRNA和蛋白表达均上调，DDR2可能在MAFLD的发生发展中发挥重要作用。
Abstract：Objective To investigate the expression changes of discoid domain receptor 2(DDR2) in the liver tissues of mice with metabolic-associated fatty liver disease(MAFLD). Methods Ten 8-week-old male C57 BL/6J mice were randomly divided into the normal chow diet group(NC group, n=5) and high-fat diet group(HFD group, n=5). The HFD group was fed a high-fat diet, while the NC group received a standard diet for 16 weeks. After 12-hour fasting, we weighed all mice and collected blood samples through orbital venipuncture to detect serum liver function indicators [alanine aminotransferase(ALT) and aspartate aminotransferase(AST)] and lipid profiles [total cholesterol(TC) and triglycerides(TG)]. Liver weight and the liver index were recorded. Enzymatic assays measured hepatic TG and free fatty acids(FFA). Hepatic histopathology was examined using HE staining and Oil Red O staining. DDR2 mRNA and protein levels in the liver tissues were detected by qPCR and Western blotting. Results Compared with the NC group, the HFD group exhibited significantly higher body weight after 16 weeks(P<0. 05). Serum ALT, AST, TC, and TG levels were higher in the HFD group than in the NC group(all P<0. 05). The liver weight, liver index, and content of liver TG and FFA in the HFD group were all higher than those in the NC group(all P<0. 01). Compared with the NC group, the HFD group exhibited marked hepatic steatosis. Both DDR2 mRNA and protein expression levels in the liver tissues were higher in the HFD group than in the NC group(both P<0. 01). Conclusion DDR2 mRNA and protein expression are up-regulated in the liver tissues of MAFLD mice, suggesting that DDR2 may play a significant role in the development and progression of MAFLD.
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基本信息:
DOI：
中图分类号:R575.5
引用信息:
[1]闪明海,胡爱丽,郭玉峰,等.代谢相关脂肪性肝病小鼠肝组织盘状结构域受体2表达变化观察[J].山东医药,2025,65(12):15-18.
基金信息:
宁夏自然科学基金项目（2021AAC03342）