山东医药

目的 探讨补中益气加味方煎剂短期灌胃对裸鼠结直肠癌细胞皮下移植瘤组织消皮素E(GSDME)表达的影响，为结直肠癌的中医治疗提供理论依据。方法 30只SPF级裸鼠采用右腿内侧皮下接种结直肠癌SW480细胞悬液的方法建立结直肠癌皮下移植瘤模型，按随机数字表法将裸鼠随机分为空白对照组、化疗组及补中益气加味方低、中、高剂量组各6只。空白对照组以0.9%NaCl灌胃，补中益气加味方低、中、高剂量组分别以26.72、53.43、106.86 g/kg补中益气加味方煎剂灌胃，化疗组腹腔注射氟尿嘧啶注射液25 mg/kg，连续给药14 d。给药期间，每日观察裸鼠活动状态及死亡情况。于给药第15天处死裸鼠，剥离移植瘤组织，测算瘤体质量及体积。采用免疫组织化学法检测结直肠癌细胞皮下移植瘤组织GSDME。结果 各组给药期间均未出现死亡现象。与空白对照组相比，化疗组裸鼠在治疗过程中表现出一定程度的活动能力下降、精神萎靡等现象，而补中益气加味方各剂量组裸鼠在治疗后精神状态良好，活动能力未见明显下降，饮食正常，一般状态优于化疗组和空白对照组。各组结直肠癌细胞皮下移植瘤质量及体积比较差异均无统计学意义（P均>0.05）。化疗组及补中益气加味方中、高剂量组结直肠癌细胞皮下移植瘤组织GSDME表达高于空白对照组、补中益气加味方低剂量组（P均<0.05）。结论 补中益气加味方煎剂短期灌胃可以提高裸鼠结直肠癌细胞皮下移植瘤组织GSDME的表达，但抑瘤效果有限。

Objective To investigate the effect of short-term intragastric administration of modified Buzhong Yiqi decoction(MBYD) on gasdermin E(GSDME) expression in subcutaneous colorectal cancer xenografts of nude mice, in order to provide theoretical basis for traditional Chinese medicine(TCM) treatment of colorectal cancer. Methods Thirty specific-pathogen-free(SPF) nude mice were subcutaneously inoculated with SW480 colorectal cancer cell suspension in the right inner thigh to establish xenograft models. Mice were randomly divided into five groups(n=6 per group): the blank control group, chemotherapy group, low-dose MBYD group, medium-dose MBYD group and high-dose MBYD group, respectively. Mice in the blank control group received 0. 9% NaCl via gavage; mice in the low-dose, mediumdose, and high-dose MBYD groups received MBYD at 26. 72, 53. 43, and 106. 86 g/kg via gavage, respectively; mice in the chemotherapy group received intraperitoneal injection of fluorouracil(25 mg/kg). All treatments lasted 14 days. Daily observations included activity status and mortality. On day 15 of administration, mice were euthanized, xenografts were excised, and tumor mass/volume were measured. Immunohistochemistry(IHC) was used to detect GSDME expression in xenograft tissues. Results No deaths occurred during treatment. Compared with the blank control group, the chemotherapy group showed reduced activity and lethargy, while all MBYD groups maintained normal mental state, activity, and diet; the general condition was better than that of the chemotherapy group and the blank control group. No significant differences in tumor mass or volume were observed among groups(all P>0. 05). GSDME expression levels were significantly higher in the chemotherapy and medium-dose and high-dose MBYD groups than in the blank control and low-dose MBYD groups(all P<0. 05). Conclusion Short-term intragastric administration of MBYD enhances GSDME expression in colorectal cancer xenografts of nude mice but exhibits limited tumor-inhibiting effects.